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USCN Life rat enzyme-linked immunosorbent assay (elisa) kits
The level of salusin-β in calcifying VSMCs and the aortas of rats. (A) Protein levels of salusin-β determined by <t>ELISA,</t> (B) mRNA levels of salusin-β, and (C, D) salusin-β protein expression in VSMCs stimulated by calcification medium at days 0, 3, 7, 10, and 14. Full blot is shown in Supplementary Figure S16. (E) Immunofluorescence staining of salusin-β in VSMCs at day 14 of stimulation. (F) Salusin-β levels in plasma, (G) mRNA level of salusin-β, and (H) protein level of salusin-β in the aortas of VC rats. (I) Immunohistochemistry staining of salusin-β in the aortas of VC rats. (J) Immunofluorescence double staining showed smooth muscle marker α-SMA (green) and salusin-β (red). Nuclei were stained by DAPI (blue). (K) Immunofluorescence double staining showed endothelial cell marker CD31 (green) and salusin-β (red). Nuclei were stained by DAPI (blue). Values are expressed as mean ± SE. *p < 0.05 versus 0 day, †p < 0.05 versus Control. n = 4–6 for each group. α-SMA, alpha smooth muscle actin; ELISA, enzyme-linked <t>immunosorbent</t> assay; mRNA, messenger RNA; SE, standard error; VC, vascular calcification; VSMCs, vascular smooth muscle cells. Color images are available online.
Rat Enzyme Linked Immunosorbent Assay (Elisa) Kits, supplied by USCN Life, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The level of salusin-β in calcifying VSMCs and the aortas of rats. (A) Protein levels of salusin-β determined by <t>ELISA,</t> (B) mRNA levels of salusin-β, and (C, D) salusin-β protein expression in VSMCs stimulated by calcification medium at days 0, 3, 7, 10, and 14. Full blot is shown in Supplementary Figure S16. (E) Immunofluorescence staining of salusin-β in VSMCs at day 14 of stimulation. (F) Salusin-β levels in plasma, (G) mRNA level of salusin-β, and (H) protein level of salusin-β in the aortas of VC rats. (I) Immunohistochemistry staining of salusin-β in the aortas of VC rats. (J) Immunofluorescence double staining showed smooth muscle marker α-SMA (green) and salusin-β (red). Nuclei were stained by DAPI (blue). (K) Immunofluorescence double staining showed endothelial cell marker CD31 (green) and salusin-β (red). Nuclei were stained by DAPI (blue). Values are expressed as mean ± SE. *p < 0.05 versus 0 day, †p < 0.05 versus Control. n = 4–6 for each group. α-SMA, alpha smooth muscle actin; ELISA, enzyme-linked <t>immunosorbent</t> assay; mRNA, messenger RNA; SE, standard error; VC, vascular calcification; VSMCs, vascular smooth muscle cells. Color images are available online.
Rat Cort Enzyme Linked Immunosorbent Assay (Elisa) Kits, supplied by DRG Instruments GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The level of salusin-β in calcifying VSMCs and the aortas of rats. (A) Protein levels of salusin-β determined by <t>ELISA,</t> (B) mRNA levels of salusin-β, and (C, D) salusin-β protein expression in VSMCs stimulated by calcification medium at days 0, 3, 7, 10, and 14. Full blot is shown in Supplementary Figure S16. (E) Immunofluorescence staining of salusin-β in VSMCs at day 14 of stimulation. (F) Salusin-β levels in plasma, (G) mRNA level of salusin-β, and (H) protein level of salusin-β in the aortas of VC rats. (I) Immunohistochemistry staining of salusin-β in the aortas of VC rats. (J) Immunofluorescence double staining showed smooth muscle marker α-SMA (green) and salusin-β (red). Nuclei were stained by DAPI (blue). (K) Immunofluorescence double staining showed endothelial cell marker CD31 (green) and salusin-β (red). Nuclei were stained by DAPI (blue). Values are expressed as mean ± SE. *p < 0.05 versus 0 day, †p < 0.05 versus Control. n = 4–6 for each group. α-SMA, alpha smooth muscle actin; ELISA, enzyme-linked <t>immunosorbent</t> assay; mRNA, messenger RNA; SE, standard error; VC, vascular calcification; VSMCs, vascular smooth muscle cells. Color images are available online.
Rat Enzyme Linked Immunosorbent Assay (Elisa) Kits, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nanjing Jiancheng Bioengineering Research Institute Co Ltd rat enzyme-linked immunosorbent assay (elisa) kits
The level of salusin-β in calcifying VSMCs and the aortas of rats. (A) Protein levels of salusin-β determined by <t>ELISA,</t> (B) mRNA levels of salusin-β, and (C, D) salusin-β protein expression in VSMCs stimulated by calcification medium at days 0, 3, 7, 10, and 14. Full blot is shown in Supplementary Figure S16. (E) Immunofluorescence staining of salusin-β in VSMCs at day 14 of stimulation. (F) Salusin-β levels in plasma, (G) mRNA level of salusin-β, and (H) protein level of salusin-β in the aortas of VC rats. (I) Immunohistochemistry staining of salusin-β in the aortas of VC rats. (J) Immunofluorescence double staining showed smooth muscle marker α-SMA (green) and salusin-β (red). Nuclei were stained by DAPI (blue). (K) Immunofluorescence double staining showed endothelial cell marker CD31 (green) and salusin-β (red). Nuclei were stained by DAPI (blue). Values are expressed as mean ± SE. *p < 0.05 versus 0 day, †p < 0.05 versus Control. n = 4–6 for each group. α-SMA, alpha smooth muscle actin; ELISA, enzyme-linked <t>immunosorbent</t> assay; mRNA, messenger RNA; SE, standard error; VC, vascular calcification; VSMCs, vascular smooth muscle cells. Color images are available online.
Rat Enzyme Linked Immunosorbent Assay (Elisa) Kits, supplied by Nanjing Jiancheng Bioengineering Research Institute Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MultiSciences Biotech Co Ltd rat il-2 enzyme-linked immunosorbent assay (elisa) kits
The level of salusin-β in calcifying VSMCs and the aortas of rats. (A) Protein levels of salusin-β determined by <t>ELISA,</t> (B) mRNA levels of salusin-β, and (C, D) salusin-β protein expression in VSMCs stimulated by calcification medium at days 0, 3, 7, 10, and 14. Full blot is shown in Supplementary Figure S16. (E) Immunofluorescence staining of salusin-β in VSMCs at day 14 of stimulation. (F) Salusin-β levels in plasma, (G) mRNA level of salusin-β, and (H) protein level of salusin-β in the aortas of VC rats. (I) Immunohistochemistry staining of salusin-β in the aortas of VC rats. (J) Immunofluorescence double staining showed smooth muscle marker α-SMA (green) and salusin-β (red). Nuclei were stained by DAPI (blue). (K) Immunofluorescence double staining showed endothelial cell marker CD31 (green) and salusin-β (red). Nuclei were stained by DAPI (blue). Values are expressed as mean ± SE. *p < 0.05 versus 0 day, †p < 0.05 versus Control. n = 4–6 for each group. α-SMA, alpha smooth muscle actin; ELISA, enzyme-linked <t>immunosorbent</t> assay; mRNA, messenger RNA; SE, standard error; VC, vascular calcification; VSMCs, vascular smooth muscle cells. Color images are available online.
Rat Il 2 Enzyme Linked Immunosorbent Assay (Elisa) Kits, supplied by MultiSciences Biotech Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The level of salusin-β in calcifying VSMCs and the aortas of rats. (A) Protein levels of salusin-β determined by ELISA, (B) mRNA levels of salusin-β, and (C, D) salusin-β protein expression in VSMCs stimulated by calcification medium at days 0, 3, 7, 10, and 14. Full blot is shown in Supplementary Figure S16. (E) Immunofluorescence staining of salusin-β in VSMCs at day 14 of stimulation. (F) Salusin-β levels in plasma, (G) mRNA level of salusin-β, and (H) protein level of salusin-β in the aortas of VC rats. (I) Immunohistochemistry staining of salusin-β in the aortas of VC rats. (J) Immunofluorescence double staining showed smooth muscle marker α-SMA (green) and salusin-β (red). Nuclei were stained by DAPI (blue). (K) Immunofluorescence double staining showed endothelial cell marker CD31 (green) and salusin-β (red). Nuclei were stained by DAPI (blue). Values are expressed as mean ± SE. *p < 0.05 versus 0 day, †p < 0.05 versus Control. n = 4–6 for each group. α-SMA, alpha smooth muscle actin; ELISA, enzyme-linked immunosorbent assay; mRNA, messenger RNA; SE, standard error; VC, vascular calcification; VSMCs, vascular smooth muscle cells. Color images are available online.

Journal: Antioxidants & Redox Signaling

Article Title: Salusin-β Promotes Vascular Calcification via Nicotinamide Adenine Dinucleotide Phosphate/Reactive Oxygen Species-Mediated Klotho Downregulation

doi: 10.1089/ars.2019.7723

Figure Lengend Snippet: The level of salusin-β in calcifying VSMCs and the aortas of rats. (A) Protein levels of salusin-β determined by ELISA, (B) mRNA levels of salusin-β, and (C, D) salusin-β protein expression in VSMCs stimulated by calcification medium at days 0, 3, 7, 10, and 14. Full blot is shown in Supplementary Figure S16. (E) Immunofluorescence staining of salusin-β in VSMCs at day 14 of stimulation. (F) Salusin-β levels in plasma, (G) mRNA level of salusin-β, and (H) protein level of salusin-β in the aortas of VC rats. (I) Immunohistochemistry staining of salusin-β in the aortas of VC rats. (J) Immunofluorescence double staining showed smooth muscle marker α-SMA (green) and salusin-β (red). Nuclei were stained by DAPI (blue). (K) Immunofluorescence double staining showed endothelial cell marker CD31 (green) and salusin-β (red). Nuclei were stained by DAPI (blue). Values are expressed as mean ± SE. *p < 0.05 versus 0 day, †p < 0.05 versus Control. n = 4–6 for each group. α-SMA, alpha smooth muscle actin; ELISA, enzyme-linked immunosorbent assay; mRNA, messenger RNA; SE, standard error; VC, vascular calcification; VSMCs, vascular smooth muscle cells. Color images are available online.

Article Snippet: Enzyme-linked immunosorbent assay The levels of salusin-β were quantified using commercially available rat enzyme-linked immunosorbent assay (ELISA) kits (USCN Life Science, Houston, TX) according to the manufacturer's instructions ( 49 ).

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Immunofluorescence, Staining, Immunohistochemistry, Double Staining, Marker